Review

dsred rab11 wt (Addgene inc)

Addgene inc is a verified supplier

About

News

Press Release

Team

Advisors

Partners

Contact

Bioz Stars

Bioz vStars

Buy from Supplier

Structured Review

Addgene inc dsred rab11 wt
Dsred Rab11 Wt, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 57 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dsred+rab11+wt/DsRed-rab11+WT+(Plasmid+%2312679)/pmc12796769-375-5-19
Average 93 stars, based on 57 article reviews

dsred rab11 wt - by Bioz Stars, 2026-11

93/100 stars

Images

other:

Article Title: A genetically-encoded fluorescence-based reporter to spatiotemporally investigate mannose-6-phosphate pathway
Article Snippet: Maintenance of a pool of active lysosomes with acidic pH and degradative hydrolases is crucial for cell health.. Abnormalities in lysosomal function are closely linked to diseases, such as lysosomal storage disorders (LSDs), neurodegeneration, intracellular infections, and cancer among others.. Emerging body of research suggests the malfunction of lysosomal hydrolase trafficking pathway to be a common denominator of several disease pathologies.

Article Title: Transmembrane protein 184B (TMEM184B) modulates endolysosomal acidification via the vesicular proton pump.
Article Snippet: FLAG-hTMEM184B-GFP was generated by Twist Biosciences.

Plasmid Preparation:

Article Title: Transmembrane protein 184B (TMEM184B) modulates endolysosomal acidification via the vesicular proton pump.
Article Snippet: FLAG-GFP was made in house. mCherry-Rab7a-7 was a gift from Michael Davidson (Addgene plasmid # 55127; http://n2t.net/addgene:55127; RRID: Addgene_55127). mCherry-Rab5a-7 was a gift from Michael Davidson (Addgene plasmid # 55126; http://n2t.net/addgene:55126; RRID: Addgene_55126). .. DsRed-rab11 WT was a gift from Richard Pagano (Addgene plasmid # 12679; http://n2t.net/addgene:12679; RRID: Addgene_12679). pLJM1-FIRE-pHLy was a gift from Aimee Kao (Addgene plasmid # 170775; http://n2t.net/addgene:170775; RRID: Addgene_170775). .. HEK293T cells were cultured in high glucose Dulbecco’s Modified Eagle Medium (DMEM; Gibco) containing 10% fetal bovine serum, 110mg/mL sodium pyruvate, and 10,000 U/mL Penicillin/Streptomycin.

Article Title: TMEM184B modulates endolysosomal acidification via the vesicular proton pump
Article Snippet: FLAG-hTMEM184B-GFP was generated by Twist Biosciences. .. DsRed-rab11 WT was Addgene plasmid #12679; RRID: Addgene_12679a (deposited by Richard Pagano). pLJM1-FIRE-pHLy was Addgene plasmid #170775; RRID: Addgene_170775 (deposited by Aimee Kao). .. mCherry-Rab7a-7 was Addgene plasmid #55127; RRID: Addgene_55127 (deposited by Michael Davidson).

Article Title: Spectrin condensates provide a nidus for assembling the axonal membrane-associated periodic skeleton
Article Snippet: .. DsRed-Rab11 WT , Choudhury et al. , Addgene Plasmid #12679. .. mRFP-Rab5 , Vonderheit et al. , Addgene Plasmid #14437.

Article Title: Neuronal endolysosomal acidification relies on interactions between transmembrane protein 184B (TMEM184B) and the vesicular proton pump
Article Snippet: FLAG-GFP was made in house. mCherry-Rab7a-7 was a gift from Michael Davidson (Addgene plasmid # 55127; http://n2t.net/addgene:55127 ; RRID: Addgene_55127). mCherry-Rab5a-7 was a gift from Michael Davidson (Addgene plasmid # 55126; http://n2t.net/addgene:55126 ; RRID: Addgene_55126). .. DsRed-rab11 WT was a gift from Richard Pagano (Addgene plasmid # 12679; http://n2t.net/addgene:12679 ; RRID: Addgene_12679). pLJM1-FIRE-pHLy was a gift from Aimee Kao (Addgene plasmid # 170775; http://n2t.net/addgene:170775 ; RRID: Addgene_170775). .. HEK293T cells were cultured in high glucose Dulbecco’s Modified Eagle Medium (DMEM; Gibco) containing 10% fetal bovine serum, 110mg/mL sodium pyruvate, and 10,000 U/mL Penicillin/Streptomycin.

Dominant Negative Mutation:

Article Title: Dynamic localization of the Na+-HCO3- co-transporter NBCn1 to the plasma membrane, centrosomes, spindle and primary cilia.
Article Snippet: Finely tuned regulation of transport protein localization is vital for epithelial function.. The Na-HCO3 co-transporter NBCn1 (also known as SLC4A7) is a key contributor to epithelial pH homeostasis, yet the regulation of its subcellular localization is not understood.. Here, we show that a predicted N-terminal β-sheet and short C-terminal α-helical motif are essential for NBCn1 plasma membrane localization in epithelial cells.

Similar Products

Image Search Results

$Role of the WIPI2 FSSS motif for recruiting WIPI2 and Retriever to Rab11 endosomes . WIPI2-knockdown HK2 cells were transfected with plasmids expressing the indicated siRNA- resistant EGFP WIPI2 variants and mCherry-RAB5, mCherry-RAB7, or DsRed-Rab11 were fixed, permeabilized, immuno-stained for VPS35L and analyzed by confocal microscopy. Examples of the quantified images are presented in Supplementary Figures S5 to S7. Colocalization with EGFP WIPI2 and VPS35L was assessed for: A. RAB11 B. RAB5 C. RAB7. Colocalization was quantified using Manders’ colocalization coefficient M2, calculated in ImageJ. M2 refers to the fraction of VPS35L colocalising with the different Rab-proteins. For the triple colocalizations WIPI2/VPS35L/RAB M2 indicates the fraction of green pixels (WIPI2) overlapping with the pixels positive for the VPS35L/RAB colocalization. Colocalization was quantified from three independent experiments in a total of 120 cells. P values were calculated applying Welch’s t-test with unequal variances. The analysis was performed with 99% confidence. ***P < 0.0001.$

Journal: bioRxiv

Article Title: CROP2, a Retriever-PROPPIN Complex Mediating Protein Export from Endosomes to the Plasma Membrane

doi: 10.1101/2025.10.08.681146

Figure Lengend Snippet: Role of the WIPI2 FSSS motif for recruiting WIPI2 and Retriever to Rab11 endosomes . WIPI2-knockdown HK2 cells were transfected with plasmids expressing the indicated siRNA- resistant EGFP WIPI2 variants and mCherry-RAB5, mCherry-RAB7, or DsRed-Rab11 were fixed, permeabilized, immuno-stained for VPS35L and analyzed by confocal microscopy. Examples of the quantified images are presented in Supplementary Figures S5 to S7. Colocalization with EGFP WIPI2 and VPS35L was assessed for: A. RAB11 B. RAB5 C. RAB7. Colocalization was quantified using Manders’ colocalization coefficient M2, calculated in ImageJ. M2 refers to the fraction of VPS35L colocalising with the different Rab-proteins. For the triple colocalizations WIPI2/VPS35L/RAB M2 indicates the fraction of green pixels (WIPI2) overlapping with the pixels positive for the VPS35L/RAB colocalization. Colocalization was quantified from three independent experiments in a total of 120 cells. P values were calculated applying Welch’s t-test with unequal variances. The analysis was performed with 99% confidence. ***P < 0.0001.

Article Snippet: Vectors expressing tagged RAB proteins were purchased from Addgene: DsRed- RAB11 (12679; deposited by R. Pagano); mCherry-RAB7 (61804; deposited by G. Voeltz); mCherry-RAB5 (49201; deposited by G. Voeltz).

Techniques: Knockdown, Transfection, Expressing, Staining, Confocal Microscopy